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The phosphorylation of Rad9-S328 fluctuates during repair of DSBs. ES-Rad9-/- cells complemented with WT and S328A-Rad9 were irradiated (5 Gy), and immediately processed (t = 0) or allowed to recovery for the indicated hours. The blots were sequentially probed with an antiserum for P-Rad9 and for actin.
