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Agarose gel of minicircle lineage-specific polymerase chain reaction (MLS-PCR) performed with some reference strains of all Trypanosoma cruzi sublineages. 1.5% agarose gel Etidium Bromide-stained. Lanes: 1) Negative Control, 2) X10 cl1 (TcI), 3) CANIII cl1 (TcIIa), 4) CBB (TcIIb), 5) IVV cl4 (TcIIb), 6) M5631 cl5 (TcIIc), 7) MNcl2 (TcIId), 8) XHCH56 (TcIId), 9) NR cl3(TcIId), 10) JGG (TcIId), 11) SC43cl1(TcIId), 12) V195 cl1(TcIIe), 13) CL Brener (TcIIe).
