Table 1.

Primer sequence and polymerase chain reaction (PCR) program for MSP1 and MSP2 amplification

Gene	Name	Primer sequences	PCR program
Msp1 primary	O1	5′ CACATGAAAGTTATCAAGAACTTGTC 3′	94°C-3 min, [94°C-25 sec, 50°C-45 sec,
	O2	5′ GTACGTCTAATTCATTTGCACG 3′	68°C-2 min] × 30, 72°C-3 min
Msp1 nested	N1	5′ GCAGTATTGACAGGTTATGG 3′	94°C-3 min, [94°C-30 sec, 50°C-45 sec,
	N2	5′ GATTGAAAGGTATTTGAC 3′	68°C-2 min] × 30, 72°C-3 min
Msp2 primary	S3	5′ GAAGGTAATTAAAACATTGTC 3′	94°C-3 min, [94°C-30 sec, 42′C-60 sec,
	S2	5′ GAGGGATGTTGCTGCTCCACAG 3′	65°C-2 min] × 30, 72°C-3 min
Msp2 nested	S1	5′ GAGTATAAGGAGAAGTATG 3′	94°C-3 min, [94°C-30 sec,
	S4	5′ CTAGAACCATGCATATGTCC 3′	50°C-60 sec, 72°C-2 min] × 45, 72°C-3 min