Table 2.
List of ISSR primers and the sequences used in this study, number of reliable bands and polymorphic bands of each primer generated and the Nm value for each primer
| Primer code | Nucleotide sequence 5′ to 3′ | No. of bands analysed | No. of polymorphic bands | Nm |
|---|---|---|---|---|
| 807 | (AG)7T | 11 | 7 | 1·0835 |
| 811 | (GA)8C | 9 | 7 | 0·8086 |
| 822 | (TC)8A | 6 | 3 | 0·7178 |
| 827 | (AC)8G | 13 | 10 | 0·5656 |
| 840 | (GA)8YT | 11 | 8 | 0·6921 |
| 855 | (AC)8YT | 9 | 7 | 1·2411 |
| 856 | (AC)8YA | 10 | 6 | 0·5735 |
| 857 | (AC)8YG | 9 | 6 | 0·6254 |
| 881 | (GGGGT)3 | 10 | 8 | 0·6103 |
| 889 | DBD(AC)7 | 7 | 3 | 1·9153 |
| 890 | VHV(GT)7 | 8 | 2 | 1·1687 |
| Total | 103 | 67 |
Y = (A, C); D = (A, G, T); B = (C, G, T); H = (A, C, T); V = (A, C, G).