. 2006 Sep;98(3):679–689. doi: 10.1093/aob/mcl141

Table 3.

Flow cytometric parameters assessed in each species

		FS (channel units)		SS (channel units)		FL (channel units)		CV (%)		BF (%)		YF (nuclei s⁻¹ mg⁻¹)
Species	Buffer	Mean	SD	Mean	SD	Mean	SD	Mean	SD	Mean	SD	Mean	SD
Sedum burrito	LB01	57.66^a	8·724	11·37^a	2·448	110·9^a	5·93	4·48^a	0·407	69·15^a	24·903	0·06^a	0·004
	Galbraith	41·06^b	5·171	5·36^b	2·299	112·1^a	2·57	3·59^ab	0·396	60·02^a	16·631	0·05^b	0·004
	Tris.MgCl₂	57.50^a	8·556	13·34^a	2·670	103·0^b	6·19	5·61^c	1·000	68·85^a	13·874	0·05^ab	0·004
	Otto	8·01^c	2·179	2·58^c	0·787	121·4^c	3·93	3·27^b	0·288	65·96^a	6·33	0·05^ab	0·006
Oxalis pes-caprae	LB01	–	–	–	–	–	–	–	–	–	–	–	–
	Galbraith	171·33^a	36·404	19·75^a	6·920	178·5^a	5·21	4·14^a	0·778	7·05^a	4·977	1·01^a	0·364
	Tris.MgCl₂	–	–	–	–	–	–	–	–	–	–	–	–
	Otto	26·32^b	4·933	3·90^b	0·641	200·7^b	6·24	3·03^b	0·415	8·96^a	5·908	0·62^b	0·125
Lycopersicon esculentum	LB01	15·42^a	2·125	2·97^a	0·494	257·9^a	11·50	2·88^a	0·880	21·21^a	14·137	2·47^a	1·512
	Galbraith	21·53^b	3·752	3·40^a	0·713	208·5^b	49·61	2·87^a	0·712	16·87^a	10·036	1·69^ab	0·814
	Tris.MgCl₂	17·48^a	4·970	4·76^b	1·562	216·7^b	27·27	3·70^b	0·836	20·52^a	12·918	1·72^ab	1·745
	Otto	3·68^c	1·537	1·95^c	0·766	269·9^a	11·17	2·18^c	0·266	28·49^b	13·254	0·91^b	0·617
Celtis australis	LB01	28·47^a	4·847	11·45^a	3·481	182·1^a	13·13	3·02^a	0·315	7·66^a	3·565	0·50^a	0·224
	Galbraith	25·57^a	1·842	4·54^b	0·637	171·3^b	7·76	2·87^a	0·259	8·27^a	3·445	0·35^a	0·162
	Tris.MgCl₂	41·71^b	6·944	16·08^c	2·157	180·3^a	10·44	2·85^a	0·419	9·10^b	5·085	0·49^a	0·291
	Otto	8·20^c	1·404	7·25^b	1·787	192·8^c	8·23	3·46^b	0·405	23·96^c	4·615	0·49^a	0·200
Pisum sativum	LB01	47·54^a	1·550	10·87^a	3·078	183·5^a	5·06	2·81^a	0·461	7·19^a	2·823	2·33^a	0·490
	Galbraith	51·97^a	3·624	5·29^b	1·155	178·6^a	3·36	3·02^ab	0·465	6·34^a	1·765	2·30^a	0·876
	Tris.MgCl₂	59·37^b	4·826	16·74^c	2·914	177·7^a	11·86	3·29^b	0·515	6·39^a	2·781	2·59^a	0·894
	Otto	5·72^c	0·993	4·79^b	1·083	190·1^a	6·06	1·94^c	0·180	9·85^b	2·668	1·15^b	0·367
Festuca rothmaleri	LB01	57·35^a	1·825	8·57^a	0·799	196·1^a	6·07	3·24^a	0·452	14·23^ab	3·283	0·42^a	0·182
	Galbraith	61·03^a	2·250	6·84^b	0·649	182·7^b	7·15	3·33^ab	0·459	15·07^a	1·974	0·27^b	0·131
	Tris.MgCl₂	69·85^a	1·603	17·72^c	0·815	185·1^b	8·06	3·66^ab	0·312	17·30^b	5·701	0·54^a	0·195
	Otto	13·84^b	2·846	8·55^a	1·603	210·9^c	6·77	3·76^b	0·613	23·86^c	12·992	0·11^c	0·066
Vicia faba	LB01	104·82^a	6·021	11·61^a	1·742	201·5^a	4·45	2·40^a	0·178	6·36^a	2·326	0·87^a	0·290
	Galbraith	114·55^b	2·787	8·58^b	1·306	196·9^a	4·88	2·41^a	0·148	5·80^a	0·806	0·82^a	0·235
	Tris.MgCl₂	115·45^b	5·194	20·36^c	1·443	191·5^a	9·10	2·91^b	0·272	6·85^a	4·609	1·30^b	0·345
	Otto	21·56^c	8·721	14·05^a	7·589	184·2^a	16·92	2·22^a	0·532	4·35^a	1·751	0·45^c	0·18

Values are given as mean and standard deviation of the mean (SD) of forward scatter (FS, channel units); side scatter (SS, channel units); fluorescence (FL, channel units); coefficient of variation of G₀/G₁ DNA peak (CV, %); background factor (BF, %) and nuclear yield factor (YF, nuclei s⁻¹ mg⁻¹). Means followed by the same letter (a, b or c) are not statistically different according to the multiple comparison Holm–Sidak test at P < 0.05. Buffer(s) that performed best in each species are shown in bold type.