Fig. 4.

Effect of desiccation (D) on mRNA level of dtGR and cGR and 18S rRNA in ‘EPACE-1’ and ‘1183’ Vigna unguiculata leaves. Desiccated leaves were air-dried at 24 °C under dim light for 0·5 h (D1), 2 h (D2) or 5 h (D3). Leaves from control plants (C) were taken prior to treatments. (A) Gel analysis of GR isoenzyme transcripts; RT-PCR was carried out on 100 ng total RNA with 35 cycles; results were visualized using a UV light transilluminator. (B) Relative mRNA level. The mRNA levels were quantified with the Imager 1D/2D software and normalized to the respective S19 gene. Relative transcript levels were calculated with reference to the controls taken as 1 (100 %). Results are means ± s.d. of three independent experiments.