Fig. 5.

Effect of exogenous abscisic acid (ABA) on mRNA level of dtGR and cGR in ‘EPACE-1’ and ‘1183’ Vigna unguiculata leaves. Exogenous ABA (100 μm) was applied by immersing petioles of excised leaves at 24 °C under dim light for 30 min (A1), 2 h (A2) and 24 h (A3). (A) Gel analysis of GR isoenzyme transcripts. RT-PCR was carried out on 100 ng total RNA with 35 cycles; results were visualized using a UV light transilluminator. (B) Relative mRNA level. The mRNA levels were quantified with the Imager 1D/2D software and normalized to the respective S19 gene. Relative transcript levels were calculated with reference to the controls taken as 1 (100 %). Results are means ± s.d. of three independent experiments.