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The HuR hinge region is essential for nuclear localization. (A) Schematic representation of wild-type and mutant HuR proteins assayed in B. (B) Subcellular localization of wild-type and truncated HuRs. NIH 3T3 cells were transiently transfected with expression vectors containing HuR-C-Flag, HuR-ΔhRRM3-C-Flag, or HuR-ΔRRM3-C-Flag, fixed with 3% paraformaldehyde for 20 min, permeabilized with 0.5% Triton X-100, and stained with monoclonal anti-Flag antibody (Sigma).
