Fig. 4.

Mean +SE cumulative 4-h food intake in animals given two injections of vehicle (white symbols), or 119 pmol NPY (A; black symbols), or 238 pmol NPY (B; black symbols) given 5 days apart (day 2 and day 7) and targeted to the LHA. A third group of animals was injected with two injections of vehicle (A; white symbols) or 119 pmol NPY (A; black symbols) given 7 days apart (day 2 and day 9). Animals were either maintained on drinking water for the duration of the experiment (EU), or maintained on drinking water (EU) and then given 2.5% saline to drink (DE). The second NPY injection was given on day 7 or day 9, 3 or 5 days after the initiation of drinking 2.5% saline. The main finding was that food intake returned to control levels when NPY was injected in the region of the LHA of DE animals showing moderate anorexia, but remained significantly suppressed in animals with a longer period of DE and more pronounced anorexia. See text for the detailed statistics.