Fig. 3.

Dynamic changes in TLR2 and TLR4 in mouse lung vascular endothelial cells (MLVECs) in response to HMGB1. MLVECs were isolated from TLR4-mutant, TLR4 WT (C3H/HeOuJ), TLR2^−/−, and TLR2 WT (C57BL/6) mice and incubated with recombinant HMGB1 (0.5 μg/ml) for up to 6 h; TLR2 and TLR4 expression in the MLVECs were then assessed using Western blot analysis. In some experiments, MyD88 inhibitory peptide (100 μM) or NF-κB inhibitor IKK-NBD (100 μM) was added to WT MLVECs 2 h prior to HMGB1. The images are representative of 3 independent studies.