Fig. 4.
Time-dependent alteration in TLRs dependency of lung activation following HS/R. WT (C3H/HeOuJ), TLR4-mutant, and TLR2−/− mice were subjected to HS/R or sham operation. Lung tissue was harvested at 2 and 8 h after resuscitation or sham operation, and activities of IRAK4 and nuclear NF-κB (A) as well as phosphorylation of p-47phox and expression of ICAM-1 (B) in the lung were detected. The graph depicts the means ± SE of the %changes, and the white bars and the black bars represent 2 h and 8 h time points, respectively. The graphs and images are representative of 3 independent studies; n = 3 mice/group. *P < 0.01 compared with sham group; **P < 0.01 compared with other groups.