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Comparing misinsertion frequencies, fins, deduced from kinetics with those measured by direct competition of dRTP vs. dWTP. The values are shown plotted as log (fins). We are able to resolve 9 of the 12 possible base substitution mispairs by direct competition, as shown in Table 1. The plots of log (fins) for (A) steady state and (B) presteady state, vs. direct dNTP competition, show linear correlations with correlation coefficients of 0.95 and 0.91, respectively.
