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. Author manuscript; available in PMC: 2010 Jan 8.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7527–7538. doi: 10.4049/jimmunol.0804121

Figure 5. The absence of Trim21 expression does not affect T-cell IL-2 production or B-cell prolifereation.

Figure 5

A, Naïve CD4+ T cells or CD8+ T cells from Trim21+/+ and Trim21−/− mice were stimulated with anti-CD3 and anti-CD28 Abs, and Trim21 mRNA was quantified by qPCR. B, IL-2 concentrations in the above cultures were measured by CBA. N.D., not detected. C, Th1 (circle)- or Th2 (square)-skewed CD4+ T cells from Trim21+/+ and Trim21−/− mice were re-stimulated with anti-CD3 plus anti-CD28 Abs or PMA plus ionomycin, and IL-2 mRNA was quantified by qPCR. D, Trim21+/+ and Trim21−/− B cells stained with CFSE were cultured with or without stimulant (LPS, CpG or anti-IgM plus anti-CD40 Abs) for 3 days. The percentages of divided cells are indicated in each panel. Data are representative of two experiments. E, Trim21+/+ and Trim21−/− mice (n = 6) were immunized with NP-KLH in alum and NP-specific IgM, IgG1, IgG2b and IgG3 Abs were detected by ELISA.