Figure 3.
Reduction of amide 15N spin-spin relaxation rate in recombinant human ubiquitin encapsulated in a reverse micelle. Spin-spin relaxation rates (1/T2) are plotted against the bulk solvent viscosity (30, 31). Data points, in order of decreasing viscosity, are for iso-octane, n-heptane, n-hexane, n-pentane, n-butane, and propane. All data were collected at 25°C. The butane and propane samples were prepared at pressures of 7 and 12 bar, respectively. All other samples were prepared at ambient pressure.