Table 2.
Tissue distribution of transcripts for gel-forming mucins in adult mice
| Relative amplicon intensity |
||||||
|---|---|---|---|---|---|---|
| Tissue | N | Muc19 | Muc5b | Muc5ac | Muc2 | Muc6 |
| Hard palate | 4 | 2 | 4 | 4 | 0 (1) | 0 (0) |
| Soft palate | 3 | 3 | 4 | 4 | 0 (1) | 0 (0) |
| Buccal mucosa | 3 | 4 | 3 | 2 | 0 (2) | 0 (0) |
| Parotid gland | 3 | 0 (0) | 0 (0) | 0 (1) | 0 (1) | 0 (0) |
| Posterior tongue | 3 | 3 | 4 | 3 | 0 (2) | 0 (0) |
| Sublingual gland | 8 | 5 | 0 (0) | 0 (0) | 0 (2) | 0 (0) |
| Sublingual mucosa | 4 | 4 | 4 | 3 | 0 (1) | 0 (0) |
| Submandibular gland | 4 | 0 (1) | 0 (1) | 0 (1) | 0 (1) | 0 (0) |
| Tracheolarynx | 4 | 1 (4) | 5 | 3 | 1 (4) | 0 (0) |
| Epididymis | 3 | 0 (0) | 3 | 1 (3) | 0 (1) | 0 (2) |
| Prostate | 3 | 0 (2) | 0 (0) | 0 (0) | 0 (ND) | ND |
| Testes | 2 | 0 (2) | 0 (1) | ND | ND | ND |
| Bulbourethral gland | 3 | 4 | 3 | 0 (1) | 0 (0) | 0 (0) |
| Ovaries/fallopian tubes | 4 | 0 (2) | 0 (3) | 1 (3) | 2 | 0 (0) |
| Uterus | 4 | 0 (1) | 0 (4) | 2 | 0 (ND) | 0 (ND) |
| Cervix | 2 | 0 (0) | 0 (3) | ND | ND | ND |
| Vagina | 2 | 0 (0) | 0 (1) | ND | ND | ND |
| Esophagus | 1 | 0 (0) | 0 (4) | ND | ND | ND |
| Gall bladder | 5 | 0 (0) | 0 (3) | 0 (3) | 0 (2) | 0 (3) |
| Stomach mucosa | 2 | 0 (0) | 1 (4) | 5 | 0 (2) | 5 |
| Duodenum | 2 | 0 (0) | 1 (3) | ND | ND | ND |
| Pancreas | 2 | 0 (0) | 0 (4) | ND | ND | ND |
| Cecum | 2 | 0 (0) | 0 (0) | ND | ND | ND |
| Jejunum | 2 | 0 (0) | 0 (0) | ND | ND | ND |
| Ileum | 2 | 0 (0) | 0 (0) | 0 | 4 | 0 (ND) |
| Colon | 2 | 0 (0) | 0 (1) | 1 (3) | 5 | 1 (2) |
| Conjunctiva | 2 | 0 (0) | 3 | 3 | 0 (ND) | 0 (ND) |
| Harderian gland | 2 | 0 (0) | 3 | 5 | 0 (2) | 0 (0) |
| Lacrimal gland | 2 | 0 (0) | 0 (0) | 2 | 0 (1) | 0 (0) |
Expression of amplicons is on a scale of 0 to 5 relative to a positive control tissue (assigned as 5). Positive controls: Muc19, sublingual glands; Muc5b, tracheolarynx; Muc5ac and Muc6, stomach; Muc2, colon. All samples (50 ng random primed cDNA) were initially assayed by PCR for 30 cycles using specific primers. Samples with relative intensities of 0 or 1 were re-assayed at 37 cycles (parentheses). N, number independent RNA/cDNA samples assayed; ND, not determined.