Figure 2.

Immunofluorescent micrographs of adult rat testis observed at low-power magnification stained for mitochondrial fatty acid β-oxidation enzymes (A–H) and CS (I), with the seminiferous epithelium at stage VII, together with differential interference contrast observation. Arrowhead, interstitial tissue; asterisk, seminiferous tubule. Note that the seminiferous epithelium is negative for the staining for mitochondrial fatty acid β-oxidation enzymes, whereas the interstitial tissue is positive for all of them. Both the interstitial tissues and seminiferous epithelial cells are positive for CS. VLCAD, very long chain acyl-CoA dehydrogenase; LCAD, long-chain acyl-CoA dehydrogenase; MCAD, medium-chain acyl-CoA dehydrogenase; SCAD, short-chain acyl-CoA dehydrogenase; MH, enoyl-CoA hydratase; HADH, 3-hydroxyacyl-CoA dehydrogenase; MTL1, 3-ketoacyl-CoA thiolase; TFP, trifunctional protein.