Figure 6.

Immunofluorescent micrographs of seminiferous tubules observed at high-power magnification, at stage V–VI, stained for mitochondrial fatty acid β-oxidation enzymes , VLCAD (A), LCAD (B), MCAD (C), SCAD (D), MH (E), HADH (F), MTL1 (G), TFP (H), and CS (I) as a marker for mitochondria (green) and tyrosinated α-tubulin (red) as a marker for Sertoli cells. Note that Sertoli cells in the seminiferous epithelium are positive for the staining for mitochondrial fatty acid β-oxidation enzymes, whereas spermatogenic cells are negative, except that both Sertoli cells and spermatogenic cells are positive for TFP. Both Sertoli cells and spermatogenic cells are positive for CS. (See Figure 2 legend for abbreviations.)