Table 4.

Manual and automated scoring of images of Hif1 IHC staining

	By pathologist					Automated
Image no.	IS	Extent-stained score	Percent stained (PS)	PS*IS	Allred score	Mean intensity (ATM)	Percent stained (PSA)
(a)	Moderate	Rare	5	0.03	4	0.16	0.03
(b)	Weak	Variable	30	0.1	4	0.26	0.33
(c)	Moderate	Variable	75	0.5	7	0.58	0.4
(d)	Weak	Variable	25	0.08	4	0.27	0.16
(e)	Strong	Uniform	100	1.0	8	0.66	0.75
(f)	Strong	Variable	25	0.25	6	0.36	0.13
(g)	Moderate	Variable	60	0.4	6	0.48	0.52
(h)	Strong	Variable	30	0.3	6	0.26	0.12
(i)	Mod/strong	Variable	35	0.29	6	0.39	0.35
(j)	Weak	Rare	2–5	0.01	3	0.21	0.03

PS and IS denote the manually measured (by pathologist) percent stained and intensity scores, respectively. PSA (percent score automated) and AI (average intensity) denote the corresponding automated scores. Hif1, hypoxia inducible factor 1; IHC, immunohistochemistry; ATM, averaged threshold measure.