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. 2010 Feb;58(2):95–107. doi: 10.1369/jhc.2009.953554

Table 5.

Manual and automated scoring of images of VEGF IHC staining

By pathologist
Automated
Image no. IS Extent-stained score Percent stained (PS) PS*IS Allred score Mean intensity (ATM) Percent stained (PSA)
(a) Moderate Variable 60 0.4 6 0.50 0.65
(b) Weak Rare/variable 15–20 0.06 4 0.37 0.15
(c) Weak/mod Rare 10–15 0.06 4 0.35 0.05
(d) Moderate Variable 40 0.27 6 0.49 0.60
(e) Strong Var/uniform 75 0.75 8 0.53 0.65
(f) Strong Variable 45 0.45 7 0.47 0.46
(g) Strong Variable 70 0.7 7 0.22 0.50
(h) Moderate Variable 45 0.3 6 0.46 0.40
(i) Strong Variable 60 0.6 7 0.57 0.64
(j) Strong Uniform 90 0.9 8 0.63 0.68

VEGF, vascular endothelial growth factor.