Figure 5.

Photograph (A) of dissected transgenic mouse onto which the measured bioluminescence image has been superimposed. The abdomen of the animal has been opened surgically and some organs have been removed to provide a clear view of its click-beetle luciferase-expressing kidneys. The bioluminescence image has been taken immediately post-mortem and following a luciferin injection. Different views of bioluminescence image reconstructions (B)–(G) of click-beetle reporter probe in kidneys prior to dissection. Reconstructions B and E show correct location of both kidneys when including the non-uniform optical property maps of segmented cyst. Conversely, reconstructions C and F show false location of left kidney (arrow) when optical property map of cyst is not included in BLT reconstruction and uniform optical property map is assumed. D and G show reconstruction results when optical property map is based on absorption coefficients of the kidney. The location of the right kidney (arrow) could not correctly be identified.