Table 3.
List of oligonucleotide primers used for RT, PCR and RACE-PCR experiments.
| GENE | ORF NUMBER | PRIMER NAME | SEQUENCE (5'-3') | POSITION ON CHROMOSOME SEQUENCE |
|---|---|---|---|---|
| unk | ECU02_0700 | 5'-unk | ATGCCATCTCGGCACTTAGAAGGCTC | complement (86499..86524) |
| 3'-unk | GCGCTCCAGCTCATCAGCGGCGAG *# | 86443..86466 | ||
| htr | ECU02_0710 | 5'-htr | ACCTGTCCGTCCATACCACGTTGC | 88604..88627 |
| 3'-htr | GATACCGACCTGGCGCTTGAACACG # | complement (87230..87254) | ||
| h2a | ECU02_0720 | 5'-h2a | TGATCTCGCTGATTAGGTACATTAC | 89217..89241 |
| 3'-h2a | GGACCAGGATGAGGATATCGAAGG | complement (89268..89291) | ||
| pap | ECU02_0730 | 5'-pap | TCAAAGAACCCACGCTCCTGTAGG | 90874..90897 |
| 3'-pap | GTCAAAGTTCGAGGCGGTTGACGAC | complement (89812..89836) | ||
| ubi | ECU02_0740i | 5'ubi | CTCGATACTGTCCGAAGGCTCGACT # | 91300..91324 |
| 3'-ubi | AGTCGAGCCTTCGGACAGTATCGAG | complement (91300..91324) | ||
| rib | ECU02_0750i | 5'-rib | TGTCATGCTCTGACAGAACCGTCC *# | complement (91758..91781) |
| 3'-rib | AGAAGAAGGTATCCAAGCTGGCC | 91698..91720 | ||
For each gene two specific primers were designed. Each of them was used for RACE-PCR. * and # characters indicate the primers used for RT and PCR, respectively. ORF number are also indicated.