Fig. 3.
Altered electrophoretic mobility of ChrXII in sgs1-ts slx4Δ strains. The indicated strains were grown to exponential phase at 25°C, arrested with α-factor and released at 37°C for the indicated times. Cells were collected and prepared for CHEF gel electrophoresis in agarose plugs. The gel was stained with ethidium bromide (top) and Southern blotted using an rDNA fragment as probe (bottom). The bottom half of the blot had no signal and is not presented. The large arrowhead indicates the position of ChrXII and the small arrowhead indicates the position of the wells.