FIGURE 5.

Mutation of a single tyrosine residue prevents the enhancement of mSlo current by α5β1 integrin activation. A, V_0.5 versus [Ca²⁺] plots from the Y766F, Y935F, and Y1027F mSlo mutants compared with WT mSlo, after integrin activation. Only the Y766F mSlo mutant failed to show a shift in its apparent Ca²⁺ sensitivity after integrin activation. B, V_0.5 versus [Ca²⁺] plots for Y766F, Y935F, and Y1027F mSlo mutants, compared with WT mSlo, after co-expression with WT Src. Only the Y766F mSlo mutant failed to show an increase in Ca²⁺ sensitivity with WT c-src co-expression.