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Geldanamycin decreases secretion of full-length ADAMTS9 and ADAMTS9 N-L2 constructs. A and B, HEK293F cells expressing ADAMTS9 (N-L2) (panel A) or full-length ADAMTS9 (panel B) were incubated with vehicle alone or geldanamycin (GA) (10 μm) for 6 h. Conditioned medium was collected, concentrated with trichloroacetic acid, and analyzed by Western blotting with the indicated antibodies.
