FIGURE 3.

Reduced phagocytosis of IgG-opsonized latex beads by MØ from CathA^S190A-Neo mice. MØ obtained from the peritoneal cavity of WT, CathA^S190A, or CathA^S190A-Neo mice were incubated for 3 h with fluorescent polystyrene latex beads opsonized or not with mouse IgG1 at 37 or 4 °C at a 1:50 ratio, washed with ice-cold PBS, fixed in 4% paraformaldehyde, and analyzed by flow cytometry. The panels show overlap of the histograms of the cells incubated with opsonized beads (solid line), non-opsonized beads (dashed line), and without the beads (gray histogram). The numbers on the panels show the average percent of beads-positive cells.