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. 2009 Oct 30;285(1):242–254. doi: 10.1074/jbc.M109.068668

FIGURE 2.

FIGURE 2.

Mutant BRAF up-regulates MAP2 promoter activity in melanoma cells. A, MAP2 promoter activity in melanoma cell lines compared with non- melanoma HeLa cells. Data shown are relative luciferase activity (RLA; mean ± S.E.; *, p < 0.001) from triplicate wells in three independent experiments. BRAF and RAS genotypes of melanoma cell lines are shown below the x axis. Q61R indicates a RAS mutation; m indicates a BRAFV600E mutation, m* indicates a BRAFV600D mutation; wt indicates the absence of NRAS or BRAF mutation in melanoma cell lines. B, mBRAF induces MAP2 mRNA in metastatic melanoma cells. RT-PCR was performed using total RNA from WM35 and 451Lu cells transfected with mBRAF. MAP2 expressions of NT2/D1 cells are shown as positive control. Actin as a control for RNA loading is shown. C, Western blot analysis of total BRAF protein expression in transiently transfected melanoma cells.