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. 2009 Oct 26;285(1):265–276. doi: 10.1074/jbc.M109.048777

FIGURE 6.

FIGURE 6.

Effect of BCATm on the allosteric regulation of GDH1 activity in the oxidative deamination reaction. A, ADP activation of GDH1 activity (○). Presence of PMP-BCATm (1:6 molar ratios) had no effect on the ADP-induced activation of GDH1. ▿, 1 molar ratio; 224, 2 molar ratio; Δ, 4 molar ratio; ·, 6 molar ratio. B, GTP is an allosteric inhibitor of GDH1 activity (○). Addition of PMP-BCATm at (▿), 1 molar ratio; (224), 2 molar ratio; Δ, 4 molar ratio; (●), 6 molar ratio antagonizes the GTP induced allosteric inhibition of GDH1. C, ribbon diagram of the two subunits of hexameric mammalian GDH1 (bovine, Protein Data Bank code 1NR7). BCATm likely competes with GTP and enhances the opening of the catalytic cleft.