TABLE 1.
Identification of metabolon proteins in extrahepatic tissues
SEQUEST was used for liquid chromatography/tandem mass spectrometry analysis, and MASCOT was used for matrix-assisted laser desorption ionization time-of-flight/time-of-flight analysis, although in most cases both were used. Data were searched against the NCBInr mammalian data base using SEQUEST using common filtering cutoff values, i.e. cross-correlation (Xcorr) values >1.8 for doubly charged ions and >2.5 for triply charged ions. In addition, ranking of preliminary score values of <5 and preliminary score values of >350 also were required for positive peptide identifications. Replicate experiments were carried out with similar results. +, present; +/−, lower content; −, absent; NC, not confirmed.
| MASCOT ID | Mitochondrial source |
||||
|---|---|---|---|---|---|
| Pancreas | Skeletal muscle | Brown adipose tissue | Heart | Kidney | |
| PC | + | +/− | + | − | + |
| E3 | + | − | + | + | + |
| GDH1 | + | + | NC | + | + |
| E2 | + | − | + | + | + |
| BCKD-E1α | + | − | + | + | + |
| BCKD-E1β | + | − | + | + | + |
| NIPSNAP1 | − | − | − | + | + |
| BDK | + | − | NC | − | + |