TABLE 3.

Inhibition constants of cathepsin V by macrocypin, clitocypin, and their mutants

Kinetic data for interaction of macrocypin 1 and cathepsin V were reported previously (9).

	10−6kon	104koff	Ki
	m−1s−1	s−1	nm
Clt	1.26 ± 0.07	1.61 ± 0.65	0.08 ± 0.03
Clt ΔG24	0.10 ± 0.06	1.8 ± 0.9	1.9 ± 0.9
Clt G24A	0.08 ± 0.01	1.2 ± 0.6	1.6 ± 0.7
Mcp1	1.48 ± 0.01	10.3 ± 0.7	0.69 ± 0.06
Mcp1 G25A	0.13 ± 0.02	16.0 ± 5.0	12.5 ± 5.2
Mcp1 ΔG25	0.15 ± 0.02	12.4 ± 2.4	8.5 ± 2.1