FIGURE 4.

Fate of prothrombin variants following cleavage by prothrombinase. Progress curves for prothrombin consumption were constructed by quantitative densitometry following fluorescence scanning of gels as illustrated in Figs. 1 and 3 and normalized as described. The data sets correspond to those describing the fate of II_WT (●), II_RR (○), II_RGR (▴), II_-1δ (▵), II_-2δ (▾), II_-3δ (▿), II_-4δ (■), and II_Q320 (□). Data points and error bars denote means ± 1 S.D. from 2–5 experiments. The lines were arbitrarily drawn. Initial rates determined from these progress curves are presented in Table 2.