FIGURE 1.

CPE sensitivity of L929 cells expressing various Cldn chimeras. A, upper panel, CPE sensitivity of the cells expressing no Cldn (filled squares), Cldn5 (filled triangles), Cldn5-4-5 (open triangles), Cldn4 (filled circles), or Cldn4-5-4 (open circles) was examined by the cytotoxicity assay with the WST-8 reagent. Lower panel, CPE sensitivity of the cells expressing no Cldn (filled squares), Cldn5 (filled triangles), Cldn5-7-5 (open triangles), Cldn7 (filled circles), or Cldn7-5-7 (open circles) was examined. The experiments were repeated three times, and representative results are shown. Each point represents the mean ± S.D. (upper panel, n = 3) or the intermediate value (lower panel, n = 2). B and C, the expression level of each Cldn was confirmed by Western blotting with anti-V5 antibody, to which 2 × 10⁴ cells/lane were subjected. The β-actin of each sample was stained as a control.