FIGURE 3.

Cldn4_FYAA functions as a receptor for CPE. A and B, CPE sensitivity (A) and ¹²⁵I-CPE-binding (B) of L929 cells expressing no Cldn (filled squares), Cldn4 (filled triangles), or Cldn4_FYAA (open triangles) were examined by cytotoxicity assay with the WST-8 reagent. The experiments were repeated twice, and representative results are shown. Each point represents the mean ± S.D. (n = 3). C, the expression level of each Cldn was confirmed by Western blotting with anti-V5 antibody, to which 2 × 10⁴ cells/lane were subjected. The β-actin of each sample was stained as a control.