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. 2009 Nov 11;285(1):401–408. doi: 10.1074/jbc.M109.051417

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FIGURE 4. — Electrostatic characteristics of the CPE-SR determines the sensitivity of Cldns to CPE. L929 cells expressing Cldns were subjected to the cytotoxicity assay (A) and ¹²⁵I-CPE binding assay (C). Cells expressing Cldn4 (filled circles), Cldn5 (filled triangles), Cldn4_NRDY (open circles), Cldn5_DYNR (open triangles), or not expressing Cldn (filled squares) were examined. Each point represents the mean ± S.D. (n = 3). B, the expression level of each Cldn was confirmed by Western blotting with anti-V5 antibody on 2 × 10⁴ cells. The β-actin of each sample was stained as a control.