TABLE 2.
CPE sensitivity of cells expressing various Cldns
Cultured cells expressing various Cldns were subjected to the cytotoxicity assay as described under “Experimental Procedures,” and the EC50 values were determined. The cells were classified into three categories as mentioned in the text. CPE-SRs are shown in bold type. The pI values are for CPE-SR. The asterisks indicate the consensus aromatic amino acids upstream of CPE-SR. mh, mouse-human chimera. mkCldn4 and huCldn1 were expressed in HEK293 cells. Other Cldns were expressed in L929 cells.
| Claudin | Amino acid sequence | pI | EC50 |
|---|---|---|---|
| ** | |||
| Highly sensitive | |||
| msCldn4 | NVIRDFYNPMVASGQKREMGAS | 9.70 | 0.21 |
| huCldn4 | NIIQDFYNPLVASGQKREMGAS | 9.70 | 0.083 |
| mkCldn4 | NIIQDFYNPLVASGQKREMGAS | 9.70 | 0.18 |
| msCldn3 | TIIRDFYNPLVPEAQKREMGAG | 6.53 | 0.20 |
| msCldn7 | QIVTDFYNPLTPMNVKYEFGPA | 6.40 | 0.29 |
| msCldn8 | SIIRDFYNPLVDVALKRELGEA | 6.49 | 0.69 |
| Slightly sensitive | |||
| msCldn14 | DVVQNFYNPLLPSGMKFEIGQA | 6.41 | 4.7 |
| msCldn2 | GILRDFYSPLVPDSMKFEIGEA | 4.18 | 4.4 |
| msCldn1 | GIVQEFYDPLTPINARYEFGQA | 4.18 | 12 |
| huCldn1 | RIVQEFYDPMTPVNARYEFGQA | 4.18 | 4.3 |
| Insensitive | |||
| msCldn5 | IVVREFYDPTVPVSQKYELGAA | 4.18 | >30 |
| huCldn5 | IVVREFYDPSVPVSQKYELGAA | 4.18 | >30 |
| huCldn10 | KITTEFFDP-LFVEQKYELGAA | 3.93 | >30 |
| Cldn mutants examined in this study | |||
| huCldn4-5-4 | NVIRDFYDPSVPVSQKYELGAS | 4.18 | >30 |
| huCldn5-4-5 | IVVREFYNPLVASGQKREMGAA | 9.70 | 0.40 |
| mhCldn7-5-7 | QIVTDFYDPSVPVSQKYELGPA | 4.18 | >30 |
| mhCldn5-7-5 | IVVREFYNPLTPMNVKYEFGAA | 6.40 | 0.13 |
| huCldn5DYNR | IVVREFYNPSVPVSQKRELGAA | 9.70 | 1.4 |
| huCldn4NRDY | NIIQDFYDPLVASGQKYEMGAS | 4.18 | 14 |