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. 2009 Nov 3;285(1):493–501. doi: 10.1074/jbc.M109.072157

FIGURE 3.

FIGURE 3.

Functional characterization of C. jejuni UNGM. A, with UDP-GalNAc as the substrate. Trace i, retention time for a standard solution of UDP-GalpNAc. Trace ii, incubation of UDP-GalpNAc with C. jejuni UNGM at equilibrium. Trace iii, standard solution of the purified product (UDP-GalfNAc) from the reaction of UDP-GalpNAc with C. jejuni UNGM. Trace iv, incubation of UDP-GalfNAc with C. jejuni UNGM at equilibrium. The same ratio of 93:7 UDP-GalpNAc to UDP-GalfNAc is seen as in trace ii. B, with UDP-Gal as the substrate. Trace i, retention time for a standard solution of UDP-Galp. Trace ii, incubation of UDP-Galp with UNGM at equilibrium. Trace iii, standard solution of UDP-Galf produced enzymatically from Galf-1-phosphate. Trace iv, incubation of UDP-Galf with C. jejuni UNGM at equilibrium. The same ratio of 93:7 UDP-Galp to UDP-Galf seen in trace ii is also observed. The peak at ∼8 min corresponds to UMP.