TABLE 1.

Kinetic parameters for the C. jejuni UNGM and mutants

Enzyme	Substrate	Km	kcat	kcat/Km	UDP-Gal:UDP-GalNAc
		μm	min−1	μm−1 min−1
Wild type	UDP-Galf	45 ± 3	178 ± 4	4.1	1.5
UNGM	UDP-GalfNAc	40 ± 6	114 ± 5	2.8
R59H	UDP-Galf	92 ± 20	590 ± 50	6.4	15
	UDP-GalfNAc	61 ± 7	26 ± 1	0.43
R168K	UDP-Galf	65 ± 9	96 ± 4	1.5	3.8
	UDP-GalfNAc	231 ± 40	89 ± 7	0.39
R59H/R168K	UDP-Galf	77 ± 9	380 ± 20	4.9	21
	UDP-GalfNAc	59 ± 6	13.8 ± 0.5	0.23
E. coli UGM	UDP-GalfNAc	NDa	NDa	NDa	NDa

^a ND means none detected.