FIGURE 1.

KC increase in MEF cells is inhibited by Tempol in WT and IKKα^−/− cells, but not in the absence of IKKβ. A, KC, the mouse homolog of IL-8, increased in the WT MEF cells after exposure to CGN. This increase was reduced by either combined exposure with the free radical scavenger Tempol (100 nm for 24 h) or silencing by BCL10 siRNA. The combination of Tempol and BCL10 silencing completely inhibited the increase in KC (p < 0.001). B, similar responses occurred in the IKKα^−/− cells (p < 0.001). C, in contrast, in the IKKβ^−/− cells, Tempol had no effect on KC secretion, demonstrating that the IKKα-mediated pathway is independent of ROS, but requires BCL10. All figures are based on at least three biological replicates with technical duplicates of each measurement. ***, p < 0.001. si, small interfering RNA; con, control.