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. 2009 Nov 6;285(1):522–530. doi: 10.1074/jbc.M109.050815

FIGURE 2.

FIGURE 2.

NF-κB family members are affected differently by CGN exposure in the IKKα−/− and IKKβ−/− cells. A, Western blot for p100 in MEF cells demonstrates no increase in the IKKα−/− cells, in contrast to changes in the IKKβ−/− and WT cells, in which p100 increases following CGN exposure and then declines following BCL10 silencing. Densitometric measurements demonstrate significant increases following CGN (p < 0.001) and significant declines when BCL10 was silenced (p < 0.001) in the WT and IKKβ−/− MEF cells, but not in the IKKα−/− MEF cells. B, in the WT cells, CGN exposure leads to significant increases in p65, p50, p52, and RelB, which are inhibited by BCL10 silencing (p < 0.001). C, in the IKKα−/− cells, no increases in p52 or RelB occur. The increases in p65 and p50 are significant and are reduced when BCL10 is silenced (p < 0.001). D, in the IKKβ−/− cells, no increases in p65 or p50 occur, and the increases in p52 and RelB are inhibited when BCL10 is silenced (p < 0.001). E, in the NCM460 cells, CGN exposure induced increases in p65, p50, p52, and RelB, but not c-Rel. These increases were inhibited when BCL10 was silenced (p < 0.001). Hence, silencing BCL10 consistently reduces the CGN-induced increases in NF-κB components.