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. 2009 Oct 30;285(1):54–70. doi: 10.1074/jbc.M109.045229

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FIGURE 5. — Identification of ASK1 phosphorylation sites on STRAP. A, purified recombinant ASK1 was assayed for its kinase activity in the presence of kinase buffer containing ∼3–4 μg of recombinant STRAP as a substrate. B, recombinant ASK1 was analyzed for its kinase activity by an in vitro kinase assay using recombinant wild-type (WT) STRAP and one of its substitution mutants, T175A/S179A, T201A/S205A, or S284A/T288A, as substrate. The phosphorylation of STRAP was also confirmed by immunoblotting (WB) with an anti-phospho-Ser/Thr antibody (second panel). re., recombinant.