FIGURE 1.

SAA induces sPLA₂ expression. A, rat smooth muscle cells were treated with (SAA) or without (control) SAA (2 μm) for 4, 8, 18, or 24 h, at which time total RNA was extracted and analyzed by real time PCR for sPLA₂ mRNA. RNA expression was calculated as described under “Experimental Procedures” and data expressed as RNA levels relative to the 0-h control ± S.D. (n = 3). B, rat smooth muscle cells were treated with (+) or without (−) SAA (4 μm) for 1, 4, or 7 days, at which time total cell protein extracts were prepared and duplicate extracts were subjected to Western blot analysis using an antiserum directed against sPLA₂. C, rat smooth muscle cells were treated with (SAA) or without (Control) SAA (2 μm) or LPS (0.1–100 ng/ml) for 24 h, at which time total RNA was extracted and analyzed by real time PCR for sPLA₂ mRNA. RNA expression was calculated as described under “Experimental Procedures” and the data expressed as RNA levels relative to the control ± S.D. (n = 3). The level of sPLA₂ mRNA in SAA-treated cells was significantly different from that in control-treated and LPS-treated samples (p < 0.003).