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Dose-dependent effects of uncouplers and valinomycin on ThTP synthesis. The DTMF was incubated (10 min, 37 °C) in the presence of Pi (5 mm), succinate (5 mm), and ThDP (1 mm) with various concentrations of CCCP (A), 2,4-dinitrophenol (DNP) (B), or valinomycin (C) (mean ± S.D., n = 2–8).
