TABLE 2.

Synthesis of ATP and ThTP in a rat brain mitochondrial fraction (DTMF) under different conditions

The mitochondria were incubated (10 min, 37 °C) in saline (cf. Table I) containing 0.2 mm ADP for ATP synthesis and 1 mm ThDP for ThTP synthesis. After centrifugation (11,000 × g, 5 min), the ThTP and ATP contents were determined in the pellet. The results are expressed as mean ± S.D., and the number of experiments is indicated in parentheses.

	[ATP]	[ThTP]
	nmol/mg of protein	pmol/mg of protein
Control	6.3 ± 2.4 (8)	2.1 ± 1.5 (4)
Pi (5 mm)	7.6 + 3.1 (9)	17 ± 3 (20)
Pi + pyruvate (5 mm)	140 ± 20 (4)	42 ± 6 (52)
Pi + pyruvate + CCCP (0.2 μm)	5.5 ± 2.7 (4)	10 ± 2 (4)
Pi + succinate (5 mm)	130 ± 50 (8)	69 ± 12 (21)
Pi + succinate + CCCP (0.2 mm)	2.4 ± 2.9 (4)	7.5 ± 0.6 (3)
Pi + succinate + valinomycin (10 nm)	2.2 ± 3.0 (4)	2.2 ± 1.9 (6)
Pi + succinate + valinomycin (1 nm)	9.0 ± 4.0 (3)	19 ± 2 (6)