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The SDI and Dpy-30 domains are required for appropriate levels of gene expression. A–D, expression of GUA1 or MDH2 was determined in the indicated strains by quantitative real-time PCR analysis. All expression analysis was relative to wild-type (WT) cells using actin (ACT1) as an internal control to normalize expression levels. Data in A–D were analyzed from four biological repeats with three technical repeats each.
