FIGURE 9.

The SDI and Dpy-30 domains are required for proper gene-specific H3K4 methylation. A and B, ChIP analysis from the indicated strains was performed using antibodies specific to H3K4 di- and trimethylation and histone H3. ChIP eluates were PCR-amplified using primer sets directed to the 5′- and 3′-ends of the open reading frames of GUA1 and MDH2 and analyzed by agarose gel electrophoresis and ethidium bromide staining. Input and histone H3 were used as loading controls. The depicted ChIP analysis is a representation of three biological repeats. WT, wild type.