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Kinetic parameters for IcmF
All experiments were performed in 50 mm NaPi, pH 7.5, 250 mm NaCl at 37 °C as described under “Experimental Procedures.” The data represent the mean ± S.D. of three independent experiments.
| Nucleotide | None | GDP | GTP |
|---|---|---|---|
| Km (isobutyryl-CoA), μm | 20.1 ± 1.3 | 45.3 ± 4.0 | 50.8 ± 2.6 |
| kcat, s−1 | 3.10 ± 0.05 | 1.96 ± 0.04 | 1.88 ± 0.08 |
| kcat/Km, m−1s−1 | (1.5 ± 0.1) × 105 | (4.3 ± 0.5) × 104 | (3.7 ± 0.4) × 104 |
