FIGURE 3.

Regions on FliN important for the interaction with FliN. A, representative gels from the GST-FliM/FliN pulldown experiments. B, results of the pulldown experiments mapped onto the structural model of the FliN tetramer. Residues important for the FliM-FliN interaction fall in two widely separated regions. The hydrophobic patch on FliN, discussed under “Discussion,” is centered on the 2-fold axis of the dimer and contains, among others, residues 111–113, which are not important for the FliM interaction. cont, control; w.t., wild-type.