TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or property | Ref. or source |
|---|---|---|
| DFB223 | fliN null strain | Ref. 35 |
| DFB228 | fliM null strain | This work |
| DFB232 | fliMN null strain | Ref. 35 |
| RP3098 | ΔflhDC; no expression of chromosomal flagellar genes | J. S. Parkinson |
| pTBM30 | Ptac expression vector; Apr; parent of pHT39 | Ref. 51 |
| pLS4 | fliN in pAlter-1; Apr | Ref. 35 |
| pHT41 | fliM in pAlter-1, vector for site-directed mutagenesis | Ref. 43 |
| pHT39 | fliN under Ptac control; Apr | Ref. 48 |
| pDFB94a | fliM under Ptac control; Cmr | This work |
| pDFB66 | cheY under Para control; Cmr | Ref. 13 |
| pHT100 | GST-only under Ptac control; Kmr | Ref. 44 |
| pHT86 | GST-FliM under Ptac control; Kmr | Ref. 44 |