FIGURE 1.

tSK2 antibody expression vector series. A, backbone of the tSK2 episomal vector. Expression cassettes are inserted between the EcoRI site and the NotI site. PCMV, CMV promoter; TPL, adenovirus 5 tripartite leader sequence; MLPenh, adenovirus major late promoter enhancer; pA, polyadenylation site. B, heavy chain and light chain expression cassettes. Sequence of the cloning sites and translation before the start of variable regions are indicated above each diagram. L, leader peptide containing its own intron; H6, hexahistidine-tag; AVI, AviTag sequence. C, primer design. In the forward primers, N … indicates the start of the variable region sequence; sequence of the reverse primers is given for chains terminating by murine JH2, JH4, JK1, or JK2.