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The effect of NPM-ALK KAL mutants on the tyrosine phosphorylation of NPM-ALK substrates. GP293 cells were transfected with HB/NPM-ALK or one of the seven HB-tagged KAL mutants. Cell lysates were harvested 24 h after gene transfection and analyzed by immunoblot to detect tyrosine phosphorylation of a number of NPM-ALK substrates. pY, phosphotyrosine; phos, phospho.
