Fig. 6.

The protection induced by the combination of anti-TGF-β and the vaccine is mediated by CD8⁺ T cells. TC1 cells (2 × 10⁴) were injected s.c. in the right flank of C57BL/6 mice. Four days after tumor challenge, some mice were immunized with the peptide vaccine as described in Fig 1. Some immunized mice were also treated i.p. with 1D11 (100 μg) every other day from the time of immunization for two weeks. Some mice were also treated with anti-CD8 after immunization as indicated. p<0.03 by Mann-Whitney test between vaccine+cont mAb group and vaccine+anti-CD8 group. All groups had 5 mice each. p<0.03 by Mann-Whitney test between vaccine+anti-TGF-β+cont mAb group and vaccine+ TGF-β+anti-CD8 group on day 29. Each data point was shown as mean ± SD. These experiments were repeated at least twice with comparable results.